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VisionBlue® (trypan blue) is a selective tissue staining agent for use as a medical aid in ophthalmic surgery. VisionBlue® (trypan blue ophthalmic solution) 0.06% is a sterile solution of trypan blue (an acid di-azo group dye). Here, we show that the pore-forming toxin HlyII of Bacillus cereus allows trypan blue staining of macrophage cells, despite the cells remaining viable and metabolically active. Trypan blue is a dye that has been widely used for selective staining of dead tissues or cells. Is trypan blue toxic?Ĭonclusions: Trypan blue is not toxic, in terms of cell viability, over an exposure time of up to 60s however, further exposure results in a gradual increase in damage of cultured human trabecular meshwork cells. For cell culture applications, trypan blue solutions are prepared in Dulbecco’s Phosphate Buffered Saline at concentration of 0.4% to 0.5%. Trypan blue is an impermeable dye that cannot pass the intact cell membrane of living cells, in contrast to dead cells’ membranes. It is the most commonly used dye in research labs to assess cell viability. Why are trypan blue and Erythrosin used to distinguish living and dead cells? The trypan blue staining assay allows for a direct identification and enumeration of live (unstained) and dead (blue) cells in a given population. Upon entry into the cell, trypan blue binds to intracellular proteins thereby rendering the cells a blue color. The dye is excluded from membrane-intact live cells, but can enter and concentrate in membrane-compromised dead cells, rendering the cells dark blue. Trypan blue has long been the gold standard for staining dead cell to determine cell viability. The trypan blue exclusion assay allows for a direct identification and enumeration of live (unstained) and dead (blue) cells in a given population. Upon entry into the cell, trypan blue binds to intracellular proteins thereby rendering the cells a bluish color. 13 How is the trypan blue exclusion assay used?.12 How is trypan blue used to quantify live cells?.11 What is the purpose of the trypan blue stain?.10 Why do dead cells take up trypan blue dye?.4 Why are trypan blue and Erythrosin used to distinguish living and dead cells?.